SPINE2 Complexes Workshop "New Strategies for cloning and expression of protein complexes in E. coli and insect cells"

Rehovot, Israel
22nd - 27th November 2009

This workshop was aimed particularly at young scientists and technical staff who want to learn at first hand new strategies for cloning and expression of protein complexes in E. coli and insect cells. The course covered LDC and LIC cloning methodologies and small-scale secreted and intracellular expression of protein complexes in E. coli and insect cells. The course was limited to 20 participants, under the auspices of the Israel Structural Proteomics Center (ISPC), headed by Joel Sussman.
Link to external site

Programme

Sunday - November 22nd

07:30 - 08:30 Breakfast at the San Martin Guesthouse

08:30 - 08:40 Welcome by the Head of the ISPC Prof. Joel L. Sussman and by the organizers Dr. Tamar Unger and Dr. Yoav Peleg 08:40 - 09:25 Strategies in DNA cloning (Yoav Peleg)

09:25 - 12:30 Session I: Cloning Restriction Free (RF)/ LDC and mutagenesis, Baculovirus infection

12:30 - 13:45 Lunch at the Jubilee Plaza Restaurant

13:45 - 14:30 Protein co expression in E. coli (Tamar Unger)

14:30 - 17:00 Session II: Cloning (continued) and transformation for protein expression

18:30 - Transportation from San Martin Guesthouse to Jaffa for Tour and Dinner

Monday - November 23rd 07:30 - 08:30 Breakfast at the San Martin Guesthouse

08:30 - 10:00Protein co expression in eukaryotic expression systems (Tamar Unger and Yoav Peleg)

10:00 - 12:30 Session III: Small scale expression in E. coli, Cloning continue

12:30 - 13:45 Lunch at the Jubilee Plaza Restaurant

13:45 - 14:30 Protein crystallization: a never ending story (Orly Dym)

14:30 - 16:30 Session IV: DNA and protein analyses tools (at the Bioinformatics Center)

16:30 - 17:30 Presentations by participants' (10 minutes each)

17:30 - 18:00 Visit to the crystallization lab at the ISPC

19:00 Dublin Pub, Rabin Science Park, Rehovot.

Tuesday - November 24th

07:30 - 08:30 Breakfast at the San Martin Guesthouse

08:30 - 09:15 Proteopedia "Life in 3D" (Jaime Prilusky)

09:15 - 12:30 Session V: Cloning, colony PCR

12:30 - 13:45 Lunch at the Jubilee Plaza Restaurant

13:45 - 14:30 Biophysical methods for detection and analysis of protein protein interaction (Gideon Schreiber)

14:30 - 16:30 Session VI: Cloning, analysis of PCR results

16:30 - 17:30 Presentations by participants' (10 minutes each)

17:30 - Free evening

Wednesday - November 25th

07:30 - 08:30 Breakfast at the San Martin Guesthouse

08:30 - 09:15 Intrinsically unstructured proteins, IUPs (Joel Sussman)

09:15 - 12:30 Session VII: Protein extraction and purification from E. coli and insect cells

12:30 - 13:45 Lunch at the Jubilee Plaza Restaurant

13:45 - 15:30 Session VIII: Protein purification (continued)

16:00 - Transportation to Jerusalem, tour by night including dinner.

Thursday - November 26th

07:30 - 08:30 Breakfast at the San Martin Guesthouse

08:30 - 09:15 Molecular Theater 3D in motion (Harry Greenblatt)

09:15 - 12:30 Session IX: SDF PAGE and Western blot analyses

12:30 - 13:45 Lunch at the Jubilee Plaza Restaurant

13:45 - 14:30 ISPC: From gene to 3D structure (Shira Albeck)

14:30 - 16:30 Session X: Western blot analyses (continued)

19:00 - Farewell dinner

Friday - November 27th

07:30 - 08:30 Breakfast at the San Martin Guesthouse

08:30 - 10:00 Discussion and Summary (Tamar Unger and Yoav Peleg)