Coexpression Meeting 13-14th Dec. 2007, York

Overview

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Please click here for the meeting program

The 1 day meeting on the present status of co-expression systems, both in E. coli and other hosts, was held on the 13th December 2007 in York, UK. The aim was to get a broad overview of experience of co–expression techniques within the Partners laboratories. The meeting assessed current status, exchanged information of the techniques currently in use, and the practical solutions to problems encountered.

Presentations

• Coexpression vector benchmarking
• The High-Throughput Expression Laboratory at York
• SPINE2C co-expression meeting Meeting
• Protein production at the NKI

payne@ysbl.york.ac.uk

Name	Affiliation	Title
Arie Geerlof	EMBL-Hamburg	Co-expression at EMBL Hamburg : present experiences and future prospects
Yoav Peleg	Weizmann	Protein Co-expression: The Israel Structural Proteomics Center (ISPC) Experience
Beata Vertessy	ENZIM, Budapest	Co-expression systems to evaluate altered tolerance of uracil - substituted DNA in Drosophila and human cells
Patrick Celie	NKI Amsterdam	Co-expression at NKI: E.coli (LIC) and insect cells experiences & summary of the benchmarking SPINE-2 effort
Elena Blagova	University of York	Adaptation of pET-YSBLIC system for co-expression of human proteins in E. coli.
Sofia Macieira	MPI Martinsreid	Heterologous expression of complexes in TLR/IL1R signalling
Imre Berger	EMBL Grenoble	Towards Eukaryotic Structural Complexomics
Thomas A. Bowden		Mammalian Expression of Protein-Protein Complexes
Ray Owens		Mammalian Expression of Protein-Protein Complexes
Arnaud Poterszman, Dider Busso		Strategies for the co-expression of complexes in E. coli and insect cells: the Strasbourg experience